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Characterization of Insulin-like Growth Factor 1 in Human Primary Brain Tumors¹

Department of Pathology [A-C. S-N., P-A. S., V. S.], Ludwig Institute of Cancer Research [V. P. C.], and Department of Neurosurgery [H. v. H.], Karolinska Hospital, Stockholm, Sweden; BioScience Center [P-A. S.], KaBI Pharmacia, Stockholm, Sweden; and Institute of Anatomy [M. R.], University of Zurich, Zurich, Switzerland

Insulin-like growth factor 1 (IGF-1) is involved in the regulation of brain development and has been suggested as an autocrine stimulator of brain tumor cell proliferation. This study demonstrates the expression of IGF-1 in tumor tissue from human gliomas and one esthesioneuroblastoma. Using immunohistochemistry, expression of an IGF-1-like peptide was localized in tumor cells of 6 of the 9 gliomas examined as well as the esthesioneuroblastoma. From one anaplastic oligodendroglioma (which showed strong IGF-1 immunostaining) the IGF-1 transcripts were characterized after isolation of mRNA followed by amplification using the reverse transcriptase-polymerase chain reaction. Two IGF-1 complementary DNAs resulting from alternative splicing of the IGF-1 primary transcript were identified. These transcripts encode two different precursor proteins which correspond to Ea IGF-1 and Eb IGF-1. The significance of IGF-1 alternative mRNA splicing pathways remains to be determined.

¹ Supported by the Swedish Medical Research Council, the Swedish Cancer Society, the Cancer Society in Stockholm, the Hans and Loo Osterman Foundation, and the Swiss National Foundation.

² To whom requests for reprints should be addressed, at Department of Pathology, Karolinska Hospital, Box 60500, S-104 01 Stockholm, Sweden.

Received 3/19/93. Accepted 4/22/93.

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